"The personnel changes we are making are part of our ongoing strategy to best position Yahoo! for revenue growth and margin expansion." a Yahoo! spokeswoman said in a statement.
"We'll continue to hire on a global basis to support our key priorities," she said.
The spokeswoman said Tuesday's layoffs concern "approximately one percent of the global employee base" at Yahoo!, which has over 13,000 employees according to the company website.
The announcement of job cuts came just hours before the Sunnyvale, California-based Yahoo! was to announce its fourth quarter results.
Yahoo! announced in December that it was cutting some 600 jobs in the third wave of layoffs at the company since late 2008.
Yahoo! has been seeking to carve out an identity as it struggles to compete with Google and Facebook on the Web.